ac-devd-afc substrate Search Results


caspase-3 assay ac-devd-afc substrate  (MBL Life science)
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MBL Life science caspase-3 assay ac-devd-afc substrate
Caspase 3 Assay Ac Devd Afc Substrate, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic fluorogenic substrates ac-devd-afc  (Bachem)
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Bachem synthetic fluorogenic substrates ac-devd-afc
Synthetic Fluorogenic Substrates Ac Devd Afc, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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substrate ac-devd-afc  (Biomol GmbH)
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Biomol GmbH substrate ac-devd-afc
Substrate Ac Devd Afc, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ac devd afc substrate assay  (GENTAUR Inc)
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GENTAUR Inc ac devd afc substrate assay
Ac Devd Afc Substrate Assay, supplied by GENTAUR Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-devd-afc+substrate/pmc04967941-155-19-22?v=GENTAUR+Inc
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caspase substrate ac-devd-afc  (Bachem)
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Bachem caspase substrate ac-devd-afc
Caspase Substrate Ac Devd Afc, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic fluorescence peptide devd-7-amino-4-trifluoromethyl coumarin (afc)  (MBL Life science)
90
MBL Life science synthetic fluorescence peptide devd-7-amino-4-trifluoromethyl coumarin (afc)
Synthetic Fluorescence Peptide Devd 7 Amino 4 Trifluoromethyl Coumarin (Afc), supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ac-devd-afc substrate  (Enzo Biochem)
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Enzo Biochem ac-devd-afc substrate
Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
Ac Devd Afc Substrate, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-devd-afc+substrate/bio_rxiv__2023__12__12__571243-132-14-16?v=Enzo+Biochem
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fluorogenic casp3 substrate ac-devd-afc  (Biomol GmbH)
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Biomol GmbH fluorogenic casp3 substrate ac-devd-afc
Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
Fluorogenic Casp3 Substrate Ac Devd Afc, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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fluorogenic substrate ac-devd-afc  (PerSeptive Biosystems Inc)
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PerSeptive Biosystems Inc fluorogenic substrate ac-devd-afc
Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
Fluorogenic Substrate Ac Devd Afc, supplied by PerSeptive Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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afc substrates ac-devd-afc and ac-deve-afc  (SM Biochemicals LLC)
90
SM Biochemicals LLC afc substrates ac-devd-afc and ac-deve-afc
Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
Afc Substrates Ac Devd Afc And Ac Deve Afc, supplied by SM Biochemicals LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-devd-afc+substrate/pmc05041198-252-5-6?v=SM+Biochemicals+LLC
Average 90 stars, based on 1 article reviews
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fluorogenic substrate ac-devd-afc  (TIB MOLBIOL)
90
TIB MOLBIOL fluorogenic substrate ac-devd-afc
Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
Fluorogenic Substrate Ac Devd Afc, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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internally quenched tetrapeptide substrate n-acetyl-aspartyl-glutamyl-valyl-aspartate-7-amino-4-trifluoromethyl coumarin ac-devd-afc  (Biomol GmbH)
90
Biomol GmbH internally quenched tetrapeptide substrate n-acetyl-aspartyl-glutamyl-valyl-aspartate-7-amino-4-trifluoromethyl coumarin ac-devd-afc
Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
Internally Quenched Tetrapeptide Substrate N Acetyl Aspartyl Glutamyl Valyl Aspartate 7 Amino 4 Trifluoromethyl Coumarin Ac Devd Afc, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-devd-afc+substrate/us07256198-1220-16-23?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
internally quenched tetrapeptide substrate n-acetyl-aspartyl-glutamyl-valyl-aspartate-7-amino-4-trifluoromethyl coumarin ac-devd-afc - by Bioz Stars, 2026-08
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Image Search Results


Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

Journal: bioRxiv

Article Title: Blocking Orai1 constitutive activity inhibits B-cell cancer migration and synergistically acts with drugs to reduce B-CLL cell survival

doi: 10.1101/2023.12.12.571243

Figure Lengend Snippet: Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

Article Snippet: As reported and described previously [ ], activity of caspase-3 was performed by using Ac-DEVD-AFC substrate (Enzo Life Sciences, France).

Techniques: Staining, Flow Cytometry, Activity Assay, Two Tailed Test